Journal: bioRxiv
Article Title: Magnetically Controlled Microrobots for In Vivo Non-Invasive Embryo Transfer
doi: 10.1101/2025.09.29.679217
Figure Lengend Snippet: (a) The whole reproductive organ harvested from the swimming microrobot-administrated mice for hematoxylin and eosin (H&E) staining analysis. (b) Confocal microscopy images of a slice view of both the uterine horns from a pseudo-pregnant mouse injected with beads and spirals loaded with beads at GD2 18:00h and evaluated at GD3 12:00h. (i) highlights the whole length uterus carrying spiral with the beads and (ii) highlights the microrobots with beads in a higher magnification image. (c) An embryo-sized bead displays a closed lumen surrounding the bead. (d) A spiral with beads showing a closed lumen surrounding the spiral. Grey: Hoechst (stains nuclei); Blue: CDH1 (stains epithelium), Pink: FOXA2 (stains glands and autofluorescence highlights beads); Red (stains blood vessels).
Article Snippet: Primary antibodies include rat anti-CDH1 (M108, Takara Biosciences; 1:500), rabbit anti-FOXA2 (ab108422, Abcam; 1:500), and Armenian-hamster anti-CD31 (AB_2161039, DSHB; 1:200).
Techniques: Staining, Confocal Microscopy, Injection